Nrog rau txoj kev loj hlob ntawm kev tshawb fawb thiab thev naus laus zis, cov cuab yeej microbiological tau maj mam hloov cov kev ua haujlwm ntawm phau ntawv, tshwj xeeb tshaj yog nyob rau hauv cov kab lis kev cai ntawm cov ntshav, automated bacterial identification, and drug rensitivity analysis, where the breakthroughs have been achieved. Txawm li cas los xij, tam sim no, lawv tsis tuaj yeem hloov cov haujlwm ntawm phau ntawv. Raws li kev cais thiab kab lis kev cai, lawv tau muab faib ua cov kab lis kev cai tsis siv neeg thiab tsis yog - kab lis kev cai.
Automated Culture Systems: Cov kab lis kev cai tsis siv neeg tau muab faib ua ob pawg tseem ceeb raws li lawv txoj haujlwm: automated blood culture detection and analysis systems and automated microbial identification and drug sensitivity analysis systems.
Automated Blood Culture Detection and Analysis Systems: Automated blood culture detection and analysis systems feem ntau kuaj pom muaj cov kab mob hauv cov ntshav. Thaum lub sijhawm kab lis kev cai, lub khoos phis tawj tshawb nrhiav thiab tshuaj xyuas qhov system cia li tshuaj xyuas kab lis kev cai fwj tsim siv cov txheej txheem tshwj xeeb. Los ntawm kev tsis tu ncua tiag tiag- lub sijhawm saib xyuas zoo tshaj li 24 teev, yog tias muaj kev hloov pauv hauv microbial kev loj hlob thiab cov metabolism, lub cuab yeej cia li ceeb toom qhia tias muaj cov kab mob.
Automated Microbial Identification and Drug Sensitivity Analysis Systems: Feem ntau, tom qab xav tias cov kab mob raug cais tawm ntawm cov qauv los ntawm kev coj noj coj ua kab lis kev cai, kev qhia phau ntawv thiab tshuaj ntsuam xyuas cov kab mob yuav tsum tau ua. Cov txheej txheem no tsis yog tsuas yog lub sijhawm-siv thiab complex, tab sis kuj raug rau ntau yam cuam tshuam. Automated microbial identification thiab tshuaj rhiab heev soj ntsuam systems hloov phau ntawv ua hauj lwm nyob rau hauv automated nrhiav thiab tsom xam. Kev txheeb xyuas cov kab mob microbial thiab tshuaj rhiab heev yog ua ib txhij. Raws li cov hauv paus ntsiab lus ntawm kev txheeb xyuas cov bioinformatics, ib qho kev qhia tshwj xeeb yog tsim. Nyob rau tib lub sijhawm, kev tshuaj xyuas cov tshuaj automated rhiab heev yog ua los ntawm kev siv disk diffusion lossis micro-cov kua txiv dilution txoj kev, ua kom txuag tau sijhawm, txo cov haujlwm ntawm phau ntawv, txhim kho kev ua haujlwm, thiab ua kom cov txiaj ntsig tau zoo.
Tsis yog - kab lis kev cai hais txog cov kab ke uas tsis xav tau kab lis kev cai. Lawv tsom mus rau kev tshawb nrhiav sai thiab muaj zog ntawm cov biomarkers tshwj xeeb, cov khoom siv ntawm tes, lossis cov metabolites hauv cov kab mob pathogenic. Lawv tsis ncaj qha ua tiav kev kuaj mob etiological sai siv biochemical, immunological, thiab molecular biological cov txheej txheem. Cov cuab yeej tseem ceeb siv yog lub ntsuas microbial ceev.
Kev kuaj xyuas biomarker: Los ntawm kev kuaj xyuas biomarkers ntawm cov kab mob kab mob, kev kis kab mob thiab tsis yog - kis kab mob tuaj yeem sib txawv, qhov hnyav ntawm tus kab mob tuaj yeem soj ntsuam tsis tu ncua, thiab kev siv cov tshuaj tiv thaiv kab mob muaj txiaj ntsig tuaj yeem coj. Cov cim tseem ceeb suav nrog procalcitonin, C-reactive protein, thiab interleukins, uas feem ntau kuaj pom siv cov txheej txheem immunoassay.
Kev kuaj pom tus kab mob: Cov neeg mob kis kab mob feem ntau muaj kub taub hau, feem ntau cuam tshuam nrog cov khoom ntawm cov kab mob nws tus kheej. Kev kuaj xyuas cov khoom ntawm cov kab mob lawv tus kheej pab txhawm rau kuaj cov kab mob los yog cov kab mob fungal. Cov txheej txheem biochemical lossis immunological feem ntau yog siv. Rau cov kab mob endotoxins, cov tswv yim xws li gel electrophoresis, colorimetry, thiab chemiluminescence yuav siv tau; rau 1,3- -D-glucan nyob rau hauv cov phab ntsa ntawm cov kab mob fungal, txoj kev xws li Limulus Amebocyte Lysate (LAL) G factor assay, ELSA, thiab immunoturbidimetry tuaj yeem siv tau; thiab rau galactomannan nyob rau hauv fungal cell phab ntsa, txoj kev xws li latex agglutination, radioimmunoassay, thiab enzyme immunoassay yuav siv tau.
Molecular biological tshawb pom ntawm nucleic acid Cheebtsam tso cai rau kev rho tawm ncaj qha ntawm DNA lossis RNA los ntawm cov qauv, yam tsis xav txog cov yam ntxwv lom ntawm cov kab mob nws tus kheej. Kev txheeb xyuas tus kab mob yog ua tiav los ntawm kev tshuaj xyuas nws cov nucleic acid ib ntus. Cov txheej txheem tshawb nrhiav feem ntau suav nrog kev nthuav dav cov noob, 16S rRNA, kev txheeb xyuas cov nucleic acid, thiab proteomics, tag nrho cov lus cog tseg zoo rau kev kuaj pom kab mob sai.
Automated blood culture systems thiab automated microbial identification and drug susceptibility testing systems yog ob lub ntsiab lus tseem ceeb hauv kev kuaj microbial. Cov kab lis kev cai ntshav tsis siv neeg siv los kuaj pom muaj cov kab mob hauv cov qauv; automated microbial identification thiab tshuaj susceptibility test systems tuaj yeem txheeb xyuas cov kab mob sib cais thiab ua hauv vitro tshuaj tiv thaiv kab mob. Tsis tas li ntawd, cov cuab yeej tshawb nrhiav cov kab mob microbial ceev uas siv tsis yog - kab lis kev cai yog siv dav.
